Comparison of the efficiency of proteins contained in lucerne hay and soya-bean meal for sheep.

I . In four feeding experiments with sheep the utilization of proteins contained in lucerne hay and of those in toasted soya-bean meal (SBM), representing a roughage and concentrate feed respectively, were examined. The ration of one group of animals contained lucerne hay as sole source of digestible protein and that of the other SBM. 2. Measurements of the nitrogen balance showed clearly the superiority of lucerne hay as protein source compared with soya-bean meal; this arose from the greater N retention found with the lucerne hay ration. 3. The influence of both rations on the metabolic pathways of the proteins was examined by following the changes of concentration of various metabolites in the rumen contents and of blood urea at different intervals after feeding. 4. Concentrations of urea found in the blood of the sheep given lucerne hay were lower than those of the other group, a finding which is in agreement with the results of the balance experiments. 5 . The concentrations of NH,-Nandof aminoacidsin the rumen liquor were higher in sheep given lucerne hay than in those given SBM. These concentrations appeared to be related to the differences between the soluble non-protein N contents of the two feeds. 6 . The results agree with the finding from in vitro experiments that rumen liquor from sheep receiving lucerne hay promoted greater synthesis of protein than rumen liquor from sheep receiving SBM. 7. Total bacterial activity as measured by the reduction of triphenyltetrazolium chloride (dehydrogenase activity) was much higher in the rumen liquor of sheep given lucerne hay than when SBM was given. 8. The concentrations of volatile fatty acids (C,-C,) were much higher in the rumen liquor in sheep on the lucerne hay diet than in those on the SBM diet. After meals of the lucerne hay diet, the concentrations of acids higher than Cz, particularly those of valeric acid, exceeded those present in the rumen liquor of the animals kept on the SBM diet. 9. Proline and alanine appeared in relatively higher concentrations in the rumen liquor than the other amino acids determined, and these two acids together with valine disappeared very rapidly. The possible conversion of these amino acids into valeric acid is considered. It is suggested that valeric acid found in relatively high concentrations in rumen liquor of sheep given lucerne hay may be due in part to the deamination of proline, which likewise disappeared very rapidly together with alanine and valine.